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Antimicrobial Agents and Chemotherapy, July 2008, p. 2632-2635, Vol. 52, No. 7
0066-4804/08/$08.00+0     doi:10.1128/AAC.01259-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Typing of SHV Extended-Spectrum β-Lactamases by Pyrosequencing in Klebsiella pneumoniae Strains with Chromosomal SHV β-Lactamase{triangledown}

Marjo Haanperä,1* Sofia D. Forssten,1 Pentti Huovinen,2 and Jari Jalava1

Laboratory of Human Microbial Ecology,1 Antimicrobial Research Laboratory, Department of Bacterial and Inflammatory Diseases, National Public Health Institute, Turku, Finland2

Received 27 September 2007/ Returned for modification 22 February 2008/ Accepted 25 April 2008

In Klebsiella pneumoniae, the cooccurrence of chromosomal and plasmid-mediated beta-lactamases can hinder their accurate molecular detection. We developed a fast and reliable method that allows the typing of isolates carrying more than one SHV gene. The method is based on pyrosequencing the DNA sequence corresponding to amino acid positions 35, 238, and 240.

* Corresponding author. Mailing address: Laboratory of Human Microbial Ecology, Department of Bacterial and Inflammatory Diseases, National Public Health Institute, Kiinamyllynkatu 13, FIN-20520 Turku, Finland. Phone: 358 2 331 6631. Fax: 358 2 331 6699. E-mail: marjo.haanpera{at}ktl.fi

{triangledown} Published ahead of print on 5 May 2008.

Antimicrobial Agents and Chemotherapy, July 2008, p. 2632-2635, Vol. 52, No. 7
0066-4804/08/$08.00+0     doi:10.1128/AAC.01259-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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