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Antimicrobial Agents and Chemotherapy, July 2008, p. 2667-2672, Vol. 52, No. 7
0066-4804/08/$08.00+0     doi:10.1128/AAC.01516-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Molecular Characterization of Enterococcus faecalis N06-0364 with Low-Level Vancomycin Resistance Harboring a Novel D-Ala-D-Ser Gene Cluster, vanL{triangledown}

David A. Boyd,1 Barbara M. Willey,2 Darlene Fawcett,3 Nazira Gillani,3 and Michael R. Mulvey1*

National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba,1 Mount Sinai Hospital and University Health Network, Toronto, Ontario,2 Rouge Valley Health Centre Centenary Site, Scarborough, Ontario, Canada3

Received 23 November 2007/ Returned for modification 13 January 2008/ Accepted 25 April 2008

Enterococcus faecalis N06-0364, exhibiting a vancomycin MIC of 8 µg/ml, was found to harbor a novel D-Ala-D-Ser gene cluster, designated vanL. The vanL gene cluster was similar in organization to the vanC operon, but the VanT serine racemase was encoded by two separate genes, vanTmL (membrane binding) and vanTrL (racemase).

* Corresponding author. Mailing address: Nosocomial Infections, Public Health Agency of Canada, 1015 Arlington St., Winnipeg, Manitoba, Canada R3E 3R2. Phone: (204) 789-2133. Fax: (204) 789-5020. E-mail: Michael_mulvey{at}phac-aspc.gc.ca

{triangledown} Published ahead of print on 5 May 2008.

Antimicrobial Agents and Chemotherapy, July 2008, p. 2667-2672, Vol. 52, No. 7
0066-4804/08/$08.00+0     doi:10.1128/AAC.01516-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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