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Antimicrob. Agents Chemother. doi:10.1128/AAC.00462-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Antifungal Resistance of Candida glabrata Vaginal Isolates and the Development of a qRT-PCR-based Azole Susceptibility Assay

Antimicrobial Resistance Division, Molecular and Cellular Biology Division, Medical Diagnostic Laboratories, L.L.C., 2439 Kuser Road, Hamilton, NJ 08690

^* To whom correspondence should be addressed. Email: sgygax{at}mdlab.com.

	Abstract

A multiplex qRT-PCR assay was developed to detect azole resistance in Candida glabrata, an important opportunistic pathogen that develops resistance rapidly. Resistance was defined as a 3-fold increase in CDR1 expression, which proved to be 100% sensitive and 95% specific upon comparison to the gold-standard microbroth dilution assay.