AAC Accepts, published online ahead of print on 2 November 2009

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Google Scholar Articles by Regev-Shoshani, G. Articles by Av-Gay, Y. PubMed PubMed Citation Articles by Regev-Shoshani, G. Articles by Av-Gay, Y.

 Previous Article  |  Next Article 

Antimicrob. Agents Chemother. doi:10.1128/AAC.00511-09
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

The slow release of Nitric Oxide from charged catheter and its effect on Biofilm formation by Escherichia coli

Gilly Regev-Shoshani, Mary Ko, Chris Miller, and Yossef Av-Gay^*

Department of Medicine, Division of Infectious Diseases, Department of Microbiology and Immunology, Department of Medicine, Division of Respiratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada; Enox Biopharma Inc. 3849 13^th Ave West. Vancouver, British Columbia, Canada

^* To whom correspondence should be addressed. Email: yossi{at}interchange.ubc.ca.

Catheter-associated urinary tract infection is the most prevalent cause of nosocomial infections. Bacteria associated with biofilm formation play a key role in the morbidity and pathogenesis of these infections. Nitric oxide (NO) is a naturally produced free radical with proven bactericidal effect. In this study, Foley urinary catheters were impregnated with gaseous NO. These catheters demonstrated a slow release of nitric oxide over a 14 day period. The charged catheters were rendered antiseptic, and as such, were able to prevent bacterial colonization and biofilm formation on their luminal and exterior surfaces. In addition, we observed that NO impregnated catheters were able to inhibit the growth of Escherichia coli within the surrounding media, demonstrating the ability to eradicate a bacterial concentration of up to 10⁴ cfu/ml.