AAC Accepts, published online ahead of print on 2 November 2009

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Antimicrob. Agents Chemother. doi:10.1128/AAC.00859-09
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

BEL-2, an expanded-spectrum ß-lactamase with increased activity toward expanded cephalosporins in Pseudomonas aeruginosa

Laurent Poirel, Jean-Denis Docquier, Filomena De Luca, Annemie Verlinde, Louis Ide, Gian Maria Rossolini, and Patrice Nordmann^*

Service de Bactériologie-Virologie, INSERM U914 "Emerging Resistance to Antibiotics", Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris-Sud, K.-Bicêtre, France; Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, Policlinico Santa Maria alle Scotte, I-53100 Siena, Italy; and Department of Microbiology, Heilig Hartziekenhuis Roeselare, Wilgenstraat 2, 8800 Roeselare, Belgium

^* To whom correspondence should be addressed. Email: nordmann.patrice{at}bct.aphp.fr.

A Pseudomonas aeruginosa isolate recovered in Belgium produced a novel extended-spectrum ß-lactamase BEL-2, differing from BEL-1 by a single Leu162Phe substitution. That modification significantly altered the kinetic properties of the enzyme, increasing its affinity for expanded-spectrum cephalosporins. The bla_BEL-2 gene was identified from a P. aeruginosa isolate clonally-related to another bla_BEL-1-positive isolate.